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Distribution of Δ 9 ‐tetrahydrocannabinol in the mouse
Author(s) -
FREUDENTHAL R. I.,
MARTIN J.,
WALL M. E.
Publication year - 1972
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1972.tb07260.x
Subject(s) - placenta , tetrahydrocannabinol , cannabinoid , spleen , endocrinology , yolk sac , medicine , chemistry , thin layer chromatography , cannabinoid receptor , biology , chromatography , biochemistry , fetus , pregnancy , receptor , embryo , antagonist , genetics , microbiology and biotechnology
Summary1 The distribution of Δ 9 ‐tetrahydrocannabinol‐ 14 C in the pregnant and nonpregnant mouse is very similar. High concentrations of radiolabel can be seen in the maternal liver, spleen, lungs, brown fat, adrenal glands, mammary glands, yolk sac placenta and corpora lutea. In the pregnant mouse Δ 9 ‐THC crosses the placenta and enters the foetuses in very low concentrations, with no apparent selective intrafoetal radiolabel accumulation sites. Autoradiograms showing the distribution of 14 C‐cannabinoid 2 h after dosing are presented. 2 A small amount of maternal liver and foetal tissue was removed from the mice, used for autoradiography and extracted with ethyl acetate. Most of the radiolabel in these tissues was solvent extractable and was separated by thin layer chromatography into two fractions, THC and metabolites. 3 It appears that most of the cannabinoid is present in a free rather than conjugated form.