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A comparison of the excitatory and inhibitory effects of non‐adrenergic, non‐cholinergic nerve stimulation and exogenously applied ATP on a variety of smooth muscle preparations from different vertebrate species
Author(s) -
BURNSTOCK G.,
SATCHELL D. G.,
SMYTHE ANNE
Publication year - 1972
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1972.tb06868.x
Subject(s) - guanethidine , cholinergic , stimulation , adrenergic , endocrinology , acetylcholine , medicine , muscle contraction , biology , ileum , inhibitory postsynaptic potential , parasympathomimetics , reserpine , contraction (grammar) , chemistry , muscarinic acetylcholine receptor , receptor
Summary1 . The responses to non‐adrenergic, non‐cholinergic nerve stimulation have been compared with those to exogenously applied ATP on seventeen different tissues from a number of vertebrate classes. 2 . Stimulation of all the mammalian gut preparations studied (with the exception of the guinea‐pig ileum) after blockade of the effects of adrenergic and cholinergic nerve stimulation by guanethidine (3·5 μ m ) and hyoscine (1–3 μ m ) caused inhibition; exogenously applied ATP mimicked this inhibitory response. 3 . Stimulation of the guinea‐pig ileum in the presence of hyoscine and guanethidine, usually caused a diphasic response, relaxation followed by contraction; exogenously applied ATP mimicked this response, in contrast to acetylcholine and noradrenaline which caused excitation and relaxation respectively. 4 . Stimulation of preparations of lower vertebrate gut and guinea‐pig bladder in the presence of hyoscine and guanethidine caused contraction; exogenously applied ATP mimicked this contractile response. 5 . In each preparation the time course of the response to ATP was similar or identical to the response to non‐adrenergic, non‐cholinergic nerve stimulation. 6 . The results are consistent with the hypothesis that a purine nucleotide may be the transmitter substance released from non‐adrenergic, non‐cholinergic nerves supplying smooth muscle preparations from a number of vertebrate classes.