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The rat isolated colon as a specific assay organ for angiotensin
Author(s) -
GAG D. J.,
SIROIS P.
Publication year - 1972
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1972.tb06851.x
Subject(s) - angiotensin ii , renin–angiotensin system , chemistry , pharmacology , antagonism , medicine , endocrinology , receptor , blood pressure
Summary1 . The antagonism of the contractile effects of prostaglandins F 2α and E 2 by polyphloretin phosphate (PPP) was studied on the rat isolated colon. 2 . PPP (20 μg/ml) was found to reduce the myotropic action of angiotensin I (10 ng/ml) and II (0·3 ng/ml) whereas larger doses of both agents were not influenced. 3 . It is concluded that when a mixture of antagonists is used, including PPP, the rat isolated colon can be used to assay angiotensin with accuracy and specificity. The addition of 8‐L‐ala‐AT II affords a further test of specificity.