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Control of pancreatic amylase release in vitro : effects of ions, cyclic AMP, and colchicine
Author(s) -
BENZ L.,
ECKSTEIN B.,
MATTHEWS E. K.,
WILLIAMS J. A.
Publication year - 1972
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1972.tb06849.x
Subject(s) - colchicine , amylase , in vitro , chemistry , endocrinology , medicine , biophysics , biochemistry , biology , enzyme
Summary1 . The time course and concentration‐response relationship of amylase release from pieces of guinea‐pig pancreas in vitro in response to bethanechol and pancreozymin was determined. 2 . Removal of Ca ++ from the medium had no effect on basal amylase release but abolished the stimulating effect on release of bethanechol. 3 . Elevation of the concentration of Mg ++ in the medium increased basal amylase release and reduced the response to bethanechol. 4 . Elevation of the concentration of K + in the medium increased amylase release; this effect was blocked by a concentration of atropine which blocked also the response to bethanechol. 5 . Cyclic AMP, dibutyryl cyclic AMP and theophylline failed to stimulate amylase release. Pancreatic cyclic AMP concentrations were found not to be increased by bethanechol, pancreozymin or an elevated concentration of K + in the medium. 6 . Colchicine had no effect on basal amylase release or the response to bethanechol or pancreozymin. 7 . It is concluded that the coupling of stimulus to secretion involves ionic control but that neither cyclic AMP production nor microtubular mechanisms play a major role in controlling exocytosis in the pancreatic acinar cell. These findings are discussed in relation to the stimulus‐secretion coupling processes in other cells.