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Pharmacology of bovine pulmonary vein anaphylaxis in vitro
Author(s) -
EYRE P.
Publication year - 1971
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1971.tb08061.x
Subject(s) - methysergide , histamine , acetylcholine , anaphylaxis , cromolyn sodium , pharmacology , bradykinin , chemistry , atropine , in vitro , guinea pig , contraction (grammar) , bronchoconstriction , serotonin , medicine , anesthesia , immunology , allergy , asthma , biochemistry , receptor
Summary1 The bovine pulmonary vein contracts in response to acetylcholine, histamine, 5‐hydroxytryptamine and bradykinin. The tissue is particularly sensitive to 5‐hydroxytryptamine (>0·1 ng/ml). Specific Schultz‐Dale reactions were elicited in the pulmonary vein in response to horse plasma. 2 The Schultz‐Dale reaction is inhibited both by antihistaminics and by the anti‐5‐hydroxytryptamine agent methysergide, but not by atropine. 3 Sodium meclofenamate inhibited anaphylactic contraction but showed a strong tendency to antagonize many agonists indiscriminately. 4 Disodium cromoglycate (DSCG: 100 μg/ml) which inhibits some immunological reactions of mast cells, and diethylcarbamazine citrate (DECC: 50 μg/ml) which inhibits slow‐reacting substance of anaphylaxis (SRS—A) elaboration, each inhibited anaphylaxis incompletely (50% or less). However, a combination of DSCG and DECC virtually abolished the Schultz‐Dale reaction in this preparation. It is tentatively suggested that the component of the anaphylactic contraction which is resistant to cromoglycate but sensitive to diethylcarbamazine could be due to SRS—A. 5 The bovine pulmonary Schultz‐Dale reaction appears to be a complex interaction of histamine, 5‐hydroxytryptamine, SRS—A and possible other agents including kinins.