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Estimation of urinary 5‐hydroxytryptamine‐ O ‐glucuronide, a metabolite of endogenous 5‐hydroxytryptamine in sheep
Author(s) -
BARTLET A. L.,
GILBERT FRANCES M.
Publication year - 1971
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1971.tb08051.x
Subject(s) - urine , glucuronide , metabolite , glucuronidase , chemistry , glucuronic acid , incubation , chromatography , xenobiotic , biochemistry , enzyme , polysaccharide
Summary1 5‐Hydroxytryptamine‐ O ‐glucuronide has been extracted from sheep urine and characterized by its chemical reactions and R F values on paper chromatograms. 2 5‐Hydroxytryptamine‐ O ‐glucuronide in urine was separated from an inhibitor of β‐glucuronidase using an anion exchange resin, and then estimated by measurement of the 5‐hydroxytryptamine (5‐HT) liberated on incubation with β‐glucuronidase. 3 About 20% of the 5‐hydroxyindoles found in sheep urine was 5‐HT conjugated with glucuronic acid (1.18 ± 0.08 mg 5‐HT/24 h, thirty experiments). 4 Sheep urine also contained free 5‐HT (0.35 ± 0.04 mg/24 h, thirty experiments) and 5‐hydroxyindolylacetic acid (4.43 ± 0.33 mg/24 h, thirty experiments). 5 5‐Hydroxytryptamine‐ O ‐sulphate was not found in sheep urine.