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Diffusion of labelled substances through isolated rat diaphragm
Author(s) -
BROOKES N.,
MACKAY D.
Publication year - 1971
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1971.tb08038.x
Subject(s) - diaphragm (acoustics) , diffusion , chemistry , biophysics , steady state (chemistry) , anatomy , diaphragm muscle , biology , respiratory system , thermodynamics , physics , acoustics , loudspeaker
Summary1 The rate of diffusion of [ 14 C] sucrose through rat diaphragm muscle is dependent on the period of preliminary soaking of the muscle in Krebs solution at 36° C. The ratio of the length of the diffusion path through the diaphragm to its geometrical thickness appears to fall from 4.39 ± 0.38 (S.E. of the mean) to 2.23 ± 0.18 after about 6 h of soaking. 2 The area of the diaphragms available for diffusion is apparently constant at 0.3–0.4 of their total area. 3 The measured thickness of the diaphragms increases by about 20% on soaking. 4 Entry of [ 131 I] iodide into the muscle fibres is detected as a fall in both steady state and non‐steady state rates of diffusion through the diaphragm. 5 Entry of [ 14 C‐methyl] decamethonium into the muscle cells is not evident from such diffusion studies in normal Krebs solution.