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Effect of paraoxon on erythrocyte metabolism as measured by oxygen uptake in vitro
Author(s) -
SANTOLUCITO J. A.,
WHITCOMB E.
Publication year - 1971
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1971.tb07111.x
Subject(s) - paraoxon , oxygen , glutathione , chemistry , parathion , in vitro , metabolism , biochemistry , cholinesterase , endocrinology , medicine , pharmacology , biology , enzyme , acetylcholinesterase , pesticide , organic chemistry , agronomy
Summary1 Oxygen consumption in vitro and persistence in the general circulation of rabbit erythrocytes treated with the cholinesterase inhibitor paraoxon were determined. 2 Paraoxon in vitro reduced oxygen consumption below a measureable level within 2 hours. By contrast, the metabolic inhibitor N‐ethylmaleimide (NEM) produced complete inhibition within 15 minutes. 3 Erythrocytes from rabbits orally dosed with parathion also exhibited marked depression of oxygen consumption. 4 Glutathione (GSH) restored oxygen uptake to pretreatment levels within 15 min in erythrocytes previously inhibited with NEM or paraoxon. 5 Erythrocytes treated with NEM were rapidly removed from the general circulation while paraoxon treated cells were removed at a rate comparable to untreated cells.