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A simple method for measuring a picogram of acetylcholine using the clam (Mya arenaria) heart
Author(s) -
COTTRELL G. A.,
POWELL B.,
STANTON M.
Publication year - 1970
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1970.tb10661.x
Subject(s) - acetylcholine , histamine , chemistry , choline , chromatography , dopamine , biochemistry , biology , pharmacology , endocrinology
Summary1 . A simple and reliable method is described for assaying 1 pg or less of acetylcholine (ACh) using a strip of clam ( Mya arenaria ) heart. 2 . Clam heart preparations generally are extremely selective for ACh. The preparation described is at least 10 5 times less sensitive to the following: acetyl coenzyme A, adrenaline, choline, dopamine, γ‐aminobutyric acid, glutamic acid, histamine, 5‐hydroxytryptamine and noradrenaline. 3 . Because this preparation is one of the most sensitive known for detecting ACh, it should prove useful for assaying particularly low levels of this substance in biological extracts.