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The assay of gastrin using the perfused rat stomach
Author(s) -
SMITH G. M.,
LAWRENCE A. J.,
COLINJONES D. G.,
SCHILD H. O.
Publication year - 1970
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1970.tb10349.x
Subject(s) - gastrin , pentagastrin , stomach , medicine , endocrinology , g cell , human stomach , chemistry , gastric acid , biology , secretion
1 A method is described for the bio‐assay of gastric secretagogues using the perfused rat stomach in which the acid output can be readily and accurately quantified. 2 This technique increases the sensitivity of the perfused rat stomach so that 10–20 ng of synthetic human gastrin I can be detected. 3 It is possible to assay secretagogues, relative to a standard with fiducial limits of less than ±30%. 4 The use of this system is illustrated by assays comparing synthetic human gastrin I with pentagastrin, pure hog gastrin and gastrin added to plasma.