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Metabolism of prostaglandin E 1 in dog kidneys
Author(s) -
NAKANO J.
Publication year - 1970
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1970.tb09924.x
Subject(s) - metabolite , chemistry , metabolism , kidney , prostaglandin e , dinoprostone , primary metabolite , chromatography , prostaglandin , biochemistry , endocrinology , biology
Summary1 The biotransformation of prostaglandin E 1 (PGE 1 ) was studied in the isolated, perfused dog kidneys. 2 An average 43% of PGE 1 was converted into the less polar metabolite I by a single passage through the kidney. As the re‐circulation of the perfusate continued, PGE 1 was converted not only into metabolite I but also the least polar metabolite II. The velocity of the conversion of PGE 1 into metabolite I was significantly greater than that into metabolite II. Usually, six passages elapsed before maximum degradation of PGE 1 occurred. 3 Further separation with silicic acid column chromatography and gas‐liquid chromatography showed that metabolite II consists of two individual metabolites, metabolite IIa and metabolite IIb. 4 The present study indicates that the kidney biotransforms PGE 1 rather rapidly into three metabolites which are less polar than PGE 1 .