Premium
Localization of kininogenase in the rat kidney
Author(s) -
NUSTAD K.
Publication year - 1970
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1970.tb09558.x
Subject(s) - microsome , differential centrifugation , kidney , chemistry , biochemistry , endoplasmic reticulum , centrifugation , alkaline phosphatase , specific activity , membrane , phosphatase , cortex (anatomy) , biology , enzyme , endocrinology , neuroscience
Summary1 . The rat kidney kininogenase (KGA) activity was located mainly in the kidney cortex. 2 . Differential centrifugation of kidney cortex homogenate revealed that the microsomal fraction contained more KGA activity per unit of protein than the other subcellular fractions. 3 . Subfractionation of the microsomal fraction showed that the KGA activity was recovered in a subfraction also containing high specific activity of both alkaline phosphate and glucose‐6‐phosphatase. It is suggested that the KGA activity is localized in the plasma membrane and/or endoplasmic reticulum membranes of kidney cortical cells. 4 . Hydrolysis of N ‐α‐benzoyl‐ l ‐arginine ethyl ester at pH 8.5 paralleled the KGA activity.