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The relationship between kidney and urinary kininogenase
Author(s) -
NUSTAD K.
Publication year - 1970
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1970.tb09557.x
Subject(s) - sephadex , chemistry , kidney , urinary system , size exclusion chromatography , urine , alkaline phosphatase , biochemistry , chromatography , arginine , renal function , endocrinology , enzyme , medicine , biology , amino acid
Summary1 . Rat kidneys which were perfused with saline contained both kininogenase (KGA) and kininase activity. These activities were separated by gel filtration on a Sephadex G‐100 column. The kininase activity was excluded from the column whereas the KGA activity was retained. Kidney KGA activity was primarily found in the sedimentable fraction of the homogenate. 2 . The kidney KGA activity was compared with the urinary KGA activity, and the following properties were found to be the same: molecular dimension, pH optimum, effect of inhibitors, and ability to liberate kinins from kininogens. 3 . A urinary sample collected over 24 h contained about 8 times the KGA activity found in the corresponding kidneys at the end of the collection period. The urine: kidney ratio for alkaline phosphatase was about 0.01. 4 . The ability of kidney and urinary samples to hydrolyse N ‐α‐benzoyl‐ l ‐arginme ethyl ester (BAEE) at pH 8.5 paralleled the KGA activity.