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Histochemical fluorescence studies on noradrenaline accumulation by Uptake 2 in the isolated rat heart
Author(s) -
CLARKE D. E.,
JONES C. J.,
LINLEY P. A.
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb09515.x
Subject(s) - phenoxybenzamine , perfusion , medicine , norepinephrine , normetanephrine , endocrinology , fluorescence microscope , chemistry , catecholamine , biology , fluorescence , dopamine , physics , quantum mechanics , propranolol
1 . The distribution of noradrenaline accumulated by Uptake 2 (Iversen, 1965) in the isolated perfused rat heart has been observed using histochemical fluorescence microscopy. 2 . On the basis of the technique used, noradrenaline seems to be predominantly associated with the cardiac muscle cells, but sympathetic nerves and other cardiac structures also serve as retention sites. 3 . Phenoxybenzamine, normetanephrine and perfusion with noradrenaline at 20° C all decreased noradrenaline accumulation. 4 . Lowered temperature significantly increased the perfusion pressure and significantly decreased the [ 14 C]‐sorbitol space in perfused hearts. 5 . The implications of these results are discussed.