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Relationship between acetylcholine synthesis and its concentration in rat cerebral cortex
Author(s) -
SHARKAWI MAHMOUD,
SCHULMAN M. P.
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb09512.x
Subject(s) - acetylcholine , incubation , chemistry , cerebral cortex , endocrinology , medicine , biophysics , biochemistry , biology
1 . The synthesis of carbon‐14 labelled acetylcholine ( 14 C‐ACh) from carbon‐14 uniformly labelled glucose (U.L. 14 C‐glucose) under different conditions has been studied. 2 . Increasing KC1 concentration from 4 m m to 31 m m was associated with a large increase in the amount of 14 C‐ACh in the medium and a significant decrease in the amount of 14 C‐ACh in the tissue. This effect was observed in slices and minces but not in homogenate. 3 . Increasing KC1 concentration from 4 m m to 31 m m resulted in the release of pre‐formed 14 C‐ACh. 4 . Elevated pre‐incubation levels of ACh in slices incubated in 4 m m KC1 medium was associated with a decrease in the formation of 14 C‐ACh. 5 . These findings are consistent with the hypothesis that the concentration of ACh in the vicinity of the site of synthesis regulates the rate of ACh formation.