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The mode of action of carbon tetrachloride on Fasciola hepatica
Author(s) -
KHALIDI A.,
ZAKI SANIHA AMIN
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb09502.x
Subject(s) - carbon tetrachloride , fasciola hepatica , mode of action , chemistry , excretion , biliary fistula , titanium tetrachloride , biochemistry , chromatography , pharmacology , biology , medicine , organic chemistry , immunology , fistula , helminths , surgery , catalysis
1 . A biliary fistula was prepared in twelve sheep. 2 . Each animal was given 2 ml. of 14 C‐carbon tetrachloride (specific activity 15 mc/m‐mole) and bile collected at 2, 4, 24 and 48 hr. 3 . Specific activity was measured by liquid scintillation counting before and after each specimen had been heated to 75°C for 10 min to drive off volatile content (carbon tetrachloride). 4 . Only traces of activity were present in the bile and heating did not alter this. 5 . There was no difference in the survival times of adult Fasciola hepatica incubated in Hedon Flieg medium with and without 25% bile from non‐medicated sheep, or with carbon tetrachloride in a concentration of 0.03%, but 25% of bile from sheep given 2 ml. of carbon tetrachloride 24 hr before greatly increased lethality in vitro . 6 . The anthelmintic action of carbon tetrachloride on F. hepatica does not result from a direct action due to excretion in bile of unchanged carbon tetrachloride but of an unidentified toxic element not necessarily derived from the drug but which arises as a result of its administration.