Premium
Partial purification of the anti‐inflammatory factor(s) in inflammatory exudate
Author(s) -
BILLINGHAM M. E. J.,
ROBINSON B. V.,
ROBSON J. M.
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb08295.x
Subject(s) - exudate , sephadex , pronase , chemistry , chromatography , anti inflammatory , size exclusion chromatography , ion chromatography , incubation , biochemistry , pharmacology , medicine , trypsin , enzyme , pathology
1 The carrageenin foot test was established as a sensitive and reliable assay procedure for determining the anti‐inflammatory activity of inflammatory exudates. 2 Incubation alone at a temperature above 70° C or with pronase at 37° C destroyed the anti‐inflammatory activity of exudate. 3 The anti‐inflammatory component of exudate was partially precipitated by 50% ammonium sulphate. 4 A partial purification process was devised using Sephadex G‐150 gel filtration and DEAE and CM cellulose ion exchange chromatography to obtain at least a 24 fold purification. 5 Measurements of 11‐hydroxycorticosteroid levels indicated that steroids were not involved in the mechanism by which the exudate produced its antiinflammatory effects.