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3 H‐Nicotine in cat superior cervical and nodose ganglia after close‐arterial injection in vivo
Author(s) -
BROWN D. A.,
HOFFMANN P. C.,
ROTH L. J.
Publication year - 1969
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1969.tb08282.x
Subject(s) - nodose ganglion , superior cervical ganglion , nicotine , cervical ganglia , ganglion , anesthesia , hexamethonium , vagus nerve , medicine , chemistry , endocrinology , anatomy , stimulation , atropine
1 Concentrations of 3 H‐nicotine in the superior cervical and nodose ganglia of anaesthetized cats were measured after close‐arterial injection. 2 Shortly after injection there was a higher concentration of 3 H‐nicotine in the superior cervical ganglion than in the nodose ganglion. Mean concentration ratios, superior cervical ganglion/nodose ganglion (S/N ratios) were: 2 min after injection, 1.60 ± 0.19; 4 min, 1.21 ± 0.19; 8 min, 0.92 ± 0.05. These ratios were independent of the dose of nicotine over the range 4 to 200 μg in 0.2 ml. 3 There was no comparable difference in the concentrations of injected 14 C‐inulin or 3 H 2 O in the two ganglia, or in total water content. 4 Procedures which reduced the pharmacological action of nicotine (pre‐treatment with hexamethonium, admixture of 14 C‐inulin) tended to reduce the S/N ratio for nicotine. 5 Autoradiographs showed that nicotine entered the neurones of both superior cervical and nodose ganglia. 6 It was concluded that the higher concentration of nicotine in the superior cervical ganglion was probably related to its selective pharmacological action at this site, and may have been due to a greater intracellular retention of nicotine.