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The effect of drugs on the constriction of isolated depolarized blood vessels in response to calcium or barium
Author(s) -
NORTHOVER B. J.
Publication year - 1968
Publication title -
british journal of pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0007-1188
DOI - 10.1111/j.1476-5381.1968.tb07062.x
Subject(s) - chemistry , calcium , perfusion , hydrochloride , cannula , papaverine , anesthesia , chromatography , medicine , biochemistry , surgery , organic chemistry
1 The rat isolated anterior mesenteric artery was perfused at a constant rate with a calcium‐free depolarizing solution. Injection close to the cannula of 0.05–0.1 ml. of solutions of CaCl 2 (117 m m ) or BaCl 2 (100 m m ) caused a rise in perfusion pressure. 2 The responses to injected CaCl 2 solution could be obtained repeatedly but those to successive injections of BaCl 2 solution slowly declined. When the responsiveness to barium had almost disappeared, it could be restored by the addition to the perfusing fluid of a small amount of calcium (0.05 m m ). 3 The contractile effects of calcium or barium were antagonized by the addition to the perfusing fluid of several anti‐inflammatory substances, certain local anaesthetics and certain spasmolytic drugs. 4 Perfusion of the mesenteric artery with a depolarizing solution containing 0.2 m m ‐CaCl 2 caused a persistent rise of the perfusion pressure. This was rapidly and completely reversed by the addition of indomethacin (4 mg/100 ml.) or cinchocaine hydrochloride (2 mg/100 ml.) to the perfusing fluid. 5 The uptake of 45 Ca by rat aorta depleted of calcium was reduced by amethocaine hydrochloride (10 mg/100 ml.) or cinchocaine hydrochloride (2 mg/100 ml.) but not by indomethacin (10 mg/100 ml.) or desipramine hydrochloride (1 mg/100 ml.).