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A SENSITIVE METHOD FOR THE ASSAY OF CATECHOL AMINES
Author(s) -
ARMITAGE A. K.,
VANE J. R.
Publication year - 1964
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1964.tb01560.x
Subject(s) - catechol , isoprenaline , chemistry , stomach , chromatography , gastric fundus , medicine , endocrinology , biochemistry , biology , stimulation
A strip of fundus from a rat's stomach was suspended in Krebs solution containing 5‐hydroxytryptamine. Movements of the muscle were recorded by means of a frontal writing lever giving a magnification of sixteen‐times. The strip relaxed when isoprenaline, adrenaline or noradrenaline was added to the organ‐bath in concentrations of 0.2 to 2 ng/ml. The preparation was most sensitive to isoprenaline and least sensitive to noradrenaline. The components of a mixture of two catechol amines could be assayed by superfusing the rat stomach and a chick rectum in the same stream of fluid.