Premium
THE SUBCELLULAR LOCALIZATION OF DOPAMINE AND ACETYLCHOLINE IN THE DOG CAUDATE NUCLEUS
Author(s) -
LAVERTY R.,
MICHAELSON I. A.,
SHARMAN D. F.,
WHITTAKER V. P.
Publication year - 1963
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1963.tb02016.x
Subject(s) - caudate nucleus , acetylcholine , dopamine , neuroscience , chemistry , nucleus , medicine , endocrinology , biology
The distribution of dopamine (3,4‐dihydroxyphènylethylamine) between the subcellular fractions from homogenates of dog caudate nucleus was compared with the distributions of acetylcholine, 5‐hydroxytryptamine and lactate dehydrogenase activity. The distributions of noradrenaline and 5‐hydroxytryptamine between the subcellular fractions from homogenates of dog hypothalamus were also determined. Most of the dopamine, in contrast to acetylcholine, occurred in the soluble supernatant fraction; the remainder was associated with the fractions rich in pinched‐off nerve ending particles, but localization in any one fraction was not as sharp as that of acetylcholine. Evidence is presented that suggests that the dopamine occurs in a free or easily released form throughout cell cytoplasm.