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ESTIMATION OF SUBSTANCE P IN MOUSE BRAIN. THE IDENTIFICATION OF INTERFERING NUCLEOTIDES IN THE EXTRACT
Author(s) -
LASZLO I.
Publication year - 1963
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1963.tb01482.x
Subject(s) - adenosine , bioassay , adenosine triphosphate , chemistry , nucleotide , substance p , ileum , adenosine monophosphate , potassium , sodium , guinea pig , adenosine diphosphate , chromatography , biochemistry , biology , medicine , endocrinology , neuropeptide , platelet , receptor , genetics , platelet aggregation , organic chemistry , gene
Substance P has been extracted from brain by previously described methods and assayed on the guinea‐pig isolated ileum. The concentrations showed a wide scatter which was not due to the bioassay or to variations in the extraction procedure. Adenosines‐monophosphate and adenosine‐5″‐triphosphate have been identified in the extracts by paper chromatography and by chemical reactions. The concentrations of these substances were sufficient to interfere with the assay of substance P, adenosine‐5″‐monophosphate diminishing and adenosine‐5″‐triphosphate increasing the contractions of the ileum due to substance P. Adenosine‐5″‐diphosphate was also detected in the extracts but its biological activity was insufficient to influence the estimations. The concentrations of adrenaline, noradrenaline, potassium ions, sodium chloride, 5‐hydroxytryptamine, and γ‐aminobutyric acid were below the threshold concentrations for interference with the assay of substance P.