Premium
PHARMACOLOGICAL RESPONSES OF THIAMINE‐DEFICIENT RAT TISSUES
Author(s) -
BOULLIN D. J.
Publication year - 1963
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1963.tb01309.x
Subject(s) - chronotropic , phrenic nerve , thiamine , acetylcholine , diaphragm (acoustics) , endocrinology , isoprenaline , medicine , physostigmine , chemistry , atropine , stimulation , cholinergic , inotrope , long term potentiation , respiratory system , heart rate , blood pressure , physics , receptor , loudspeaker , acoustics
Rats were rendered deficient in thiamine by feeding a synthetic diet free of the vitamin. Responses of the isolated heart to acetylcholine, adrenaline, noradrenaline and isoprenaline, and of the phrenic nerve‐diaphragm preparation to tubocurarine, gallamine and eserine, were compared with responses of tissues obtained from litter‐mate rats pair‐fed an identical diet with the addition of 25 μg of thiamine hydrochloride per day. In thiamine‐deficient tissues eserine failed to produce sustained potentiation of the twitch response of the phrenic nerve‐diaphragm preparation to single supramaximal nerve stimuli. The perfused thiamine‐deficient heart was more sensitive to acetylcholine, adrenaline, noradrenaline and isoprenaline, which produced greater negative or lesser positive chronotropic and inotropic effects. There was no significant difference in the response of the phrenic nerve‐diaphragm preparation to tubocurarine and gallamine, or to eserine with faradic stimulation.