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NORADRENALINE CONTENT IN THE HEART AND SPLEEN OF THE MOUSE UNDER NORMAL CONDITIONS AND AFTER ADMINISTRATION OF SOME DRUGS
Author(s) -
SHARMAN D. F.,
VANOV S.,
VOGT MARTHE
Publication year - 1962
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1962.tb01458.x
Subject(s) - spleen , histamine , chemistry , iproniazid , nicotine , reserpine , endocrinology , medicine , catechol , norepinephrine , ether , dopamine , pharmacology , biochemistry , enzyme , monoamine oxidase , organic chemistry
The noradrenaline content of the heart and spleen was investigated in normal mice and in mice treated with drugs. A modification of the methods of Bertler, Carlsson & Rosengren (1958) was used for extraction, and of v. Euler & Floding (1955) for fluorimetric estimation of the amine. In normal mice the mean noradrenaline content of the heart was 0.55 μg/g and that of the spleen 0.26 μg/g fresh tissue. Iproniazid (100 mg/kg), nicotine (0.1 mg/kg) and histamine (0.5 mg/kg), given 1 and 3 hr before killing the mice, did not significantly change the concentration of noradrenaline in the heart. Neither did nicotine and histamine, administered 1 hr before death, significantly alter the noradrenaline content of the spleen. The rapid changes in the catechol amine content of mouse tissues reported with these drugs by De Schaepdryver & Preziosi (1959) were not observed. In contrast, reserpine (2.5 mg/kg), methyl reserpate methyl ether (1 mg/kg), and methyl 18‐epireserpate methyl ether (2 mg/kg) caused severe depletion of noradrenaline from the heart and spleen of the mice.