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PHARMACOLOGICAL ACTIONS OF PEPSITENSIN
Author(s) -
BLAIR A. M. J. N.
Publication year - 1962
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1962.tb01406.x
Subject(s) - chemistry , antidiuretic , incubation , pepsin , endocrinology , medicine , tyramine , renin–angiotensin system , plasma renin activity , angiotensin ii , ileum , blood pressure , biochemistry , hormone , receptor , biology , enzyme
In confirmation of the results of Croxatto and his co‐workers, plasma proteins incubated with pepsin yielded a substance (pepsitensin) with pressor activity. Euglobulin gave a much higher yield than the other plasma protein fractions. Incubation for 4 hr at p H 4 gave high yields of pressor activity (pepsitensin) but no antidiuretic activity; incubation of euglobulin for 4 hr at p H 2.5 yielded extracts with antidiuretic as well as pressor activity. Incubation for 8 to 11 hr at p H 2.5 produced the highest yield of both activities. Further incubation, at the same p H, up to 20 hr caused a rapid decline in the pressor activity of the extracts, but the antidiuretic activity was much more resistant to destruction by pepsin. Pepsitensin was found to be very soluble in water and poorly soluble in organic solvents. It is not inactivated by thioglycollate. In blood pressure assays some animals did not respond to pepsitensin, and nephrectomized (17 to 24 hr) rats were found to be more suitable preparations. Pepsitensin was shown to exert pressor effect by direct action on the blood vessels. Its pressor action could be differentiated from that of tyramine, dimethylphenylpiperazine, nicotine, noradrenaline and Pitressin but not from that of angiotensin. The isolated guinea‐pig ileum and the rat uterus were equally sensitive to angiotensin and pepsitensin. In paper chromatograms, in the solvent system butanol‐acetic acid‐water, the R F of pepsitensin was very similar to that of angiotensin.