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A NOTE ON THE DISTRIBUTION OF [ 14 C]‐HISTAMINE ADDED TO BLOOD
Author(s) -
LINDELL S.E.,
VISKE K.
Publication year - 1961
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1961.tb01113.x
Subject(s) - histamine , in vitro , chemistry , whole blood , endocrinology , fraction (chemistry) , medicine , blood plasma , human blood , blood cell , immunology , biology , biochemistry , chromatography , physiology
14 C‐Labelled histamine was incubated with canine or human blood at 37° C. After 1 hr 80 to 100% of the added histamine could be recovered in unchanged form. When [ 14 C]‐histamine was added to whole blood in vitro it tended to become equally distributed between the cell and plasma fraction of the blood, and when the cell fraction of blood containing [ 14 C]‐histamine was suspended in [ 14 C]‐histamine‐free plasma the labelled histamine tended to become equally distributed between cells and plasma. The pattern of distribution of intravenously injected [ 14 C]‐histamine in the blood of anaesthetized dogs seemed to be the same as that of histamine added in vitro . The injected histamine entered the cell fraction of the blood at a slow rate. These experiments indicate that in dog and man the blood cells are of little or no importance for the inactivation of histamine released into the circulating blood.