Premium
THE DISTRIBUTION OF 5‐HYDROXYTRYPTAMINE AND ADENOSINETRIPHOSPHATE IN CYTOPLASMIC PARTICLES OF THE DOG'S SMALL INTESTINE
Author(s) -
PRUSOFF W. H.
Publication year - 1960
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1960.tb00274.x
Subject(s) - chemistry , catechol , medulla , succinic dehydrogenase , cytoplasm , amine gas treating , succinate dehydrogenase , small intestine , biophysics , biochemistry , mitochondrion , chromatography , anatomy , enzyme , biology , organic chemistry
The distribution of 5‐hydroxytryptamine, adenosinetriphosphate, and succinic dehydrogenase in sucrose homogenates of the dog's small intestine has been studied. The adenosinetriphosphate was present in two different layers which could be separated by density gradient centrifugation. The upper layer contained also much succinic dehydrogenase, but no amine; it is probably composed of mitochondria. The lower layer contained not only adenosinetriphosphate but also the major portion of the particle‐held 5‐hydroxytryptamine. The mean molar ratio, amine: adenosinetriphosphate, in the lower layer was 2.6. The experiments suggest that adenosinetriphosphate in the intestine is of importance in the storage of 5‐hydroxytryptamine, resembling the function of adenosinetriphosphate in the storage of the catechol amines of the adrenal medulla.