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A METHOD FOR MEASURING THE ACTIVITY OF COMPOUNDS WITH AN ACTIVITY LIKE VITAMIN K AGAINST INDIRECT ANTICOAGULANTS IN RATS
Author(s) -
LOWENTHAL J.,
TAYLOR J. D.
Publication year - 1959
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1959.tb00922.x
Subject(s) - vitamin , vitamin k , reproducibility , chemistry , chromatography , medicine , endocrinology , biochemistry
A method for the estimation of the activity of compounds like vitamin K using rats treated with an indirect anticoagulant is described. The assay measured the speed of onset and magnitude of the antidotal effect. Under the conditions employed, only vitamin K 1 produced a significant response. Vitamin K 3 and its water‐soluble derivatives were inactive. Both the magnitude and the speed of onset of the antidotal effect of vitamin K 1 were found to depend on the extent to which it was in solution. The assay gave results of fair accuracy and reproducibility, which permitted a full statistical analysis and provided an estimate of error.