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A SENSITIVE METHOD FOR THE ASSAY OF 5‐HYDROXYTRYPTAMINE
Author(s) -
VANE J. R.
Publication year - 1957
Publication title -
british journal of pharmacology and chemotherapy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.432
H-Index - 211
eISSN - 1476-5381
pISSN - 0366-0826
DOI - 10.1111/j.1476-5381.1957.tb00146.x
Subject(s) - acetylcholine , histamine , chromatography , chemistry , fundus (uterus) , ergonovine , uterus , serotonin , stomach , pharmacology , endocrinology , medicine , biochemistry , surgery , receptor , myocardial infarction , angina
The movements of a strip of fundus from a rat stomach, suspended in a 5 ml. bath at 37°, are recorded by a spring‐loaded lever. The muscle is stretched for 15 to 30 sec. between contractions. 1 ng. 5‐Hydroxytryptamine (5‐HT), 10 ng. acetylcholine or 2 to 10 μg. of histamine all produce contractions, giving tracings about 4 cm. high. In the presence of hyoscine (10 −7 ) low concentrations of 5‐HT can be assayed directly from mixtures of the three substances. Sympathomimetic amines interfere with the assay, but this can be overcome. The preparation is robust, potentially suitable for use with an automatic assay apparatus and 10 to 100 times more sensitive than the rat uterus.