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Assessment of acute moderate hyperglycemia on traditional and thromboelastometry coagulation parameters in healthy adult horses
Author(s) -
McGovern Kate F.,
Lascola Kara M.,
Smith Stephanie A.,
ClarkPrice Stuart C.,
McMichael Maureen,
Wilkins Pamela A.
Publication year - 2012
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/j.1476-4431.2012.00792.x
Subject(s) - medicine , partial thromboplastin time , thromboelastometry , prothrombin time , anesthesia , fibrinogen , catheter , antithrombin , thrombin time , coagulation , prospective cohort study , surgery , heparin
Objective To determine whether experimentally induced acute moderate hyperglycemia is associated with coagulation activation in healthy adult horses. Design Prospective experimental study. Setting University veterinary teaching hospital. Animals Six healthy adult horses. Interventions Hyperglycemia (10.0–13.3 mmol/L [180–240 mg/dL]) was induced and maintained for 6 hours using a hyperglycemic clamp technique. Blood glucose concentrations were assessed using a point of care ( POC ) glucometer at 10‐ and 20‐minute intervals throughout the hyperglycemic clamp procedure. Platelet count, fibrinogen concentration, prothrombin time ( PT ), activated partial thromboplastin time (a PTT ), thrombin antithrombin complex level ( TAT ), and thromboelastometry ( TEM ) were determined before and after jugular catheter placement, prior to glucose administration, and at 3 and 6 hours of sustained hyperglycemia. Data were analyzed by repeated measures analysis of variance with significance defined as P < 0.05. Measurements and Main Results All horses maintained blood glucose concentration >10.0 mmol/L (>180 mg/dL) throughout the duration of the hyperglycemic clamp with a mean concentration of 11.9 ± 0.3 mmol/L [216 ± 6 mg/dL] as measured by the POC glucometer. No significant difference was found for any evaluated parameter associated with sustained hyperglycemia. Intravenous catheter placement resulted in a significant increase in mean TAT (0.8 ± 0.3 μg/L pre‐catheter, 2.3 ± 0.8 μg/L post‐catheter; P = 0.008). Conclusions Acute, moderate hyperglycemia in healthy adult horses does not have a detectable effect on coagulation based on evaluated parameters. Jugular catheter placement results in a transient increase in thrombin generation as determined by increased TAT concentrations.