Premium
Effects of rest temperature, contact activation, and sample technique on canine thrombelastography
Author(s) -
Ralph Alan G.,
Brainard Benjamin M.,
Pittman Jennifer R.,
Babski Danielle M.,
Koenig Amie
Publication year - 2012
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/j.1476-4431.2012.00730.x
Subject(s) - venipuncture , medicine , syringe , thrombelastography , fibrinolysis , anesthesia , nuclear medicine , zoology , platelet , biology , psychiatry
Objective To determine the effects of rest temperature, contact activation ( CA ), and sample collection technique on thrombelastography ( TEG ) using canine whole blood. Design Prospective, experimental study. Setting University‐based research facility. Animals Twelve healthy, adult, mixed‐breed dogs. Interventions Blood was collected by jugular venipuncture. Tubes containing 3.2% sodium citrate, with and without 75 μg/mL corn trypsin inhibitor ( CTI ), were filled by vacuum. Samples rested for 30 minutes at 3 temperatures: 37°C, room temperature ( RT , 20–22°C), or warmed to 37°C 5 minutes prior to analysis (prewarmed). Samples were analyzed at 37°C. CTI ‐treated samples were analyzed with and without 1:50,000 tissue factor ( TF ) as activator. Six dogs were also tested similarly using a needle/syringe collection technique. Measurement and Main Results Prewarmed samples exhibited greater MA compared to RT (55.5 ± 7.2 mm vs. 53.5 ± 6.0, P < 0.05), while 37°C samples exhibited a steeper angle (56.7 ± 10.4°C vs. 52.4 ± 8.6°C) and greater MA (55.9 ± 7.5 mm vs. 53.5 ± 6.0 mm) than RT samples (both P < 0.05). CTI ‐treated samples were hypocoagulable ( R time 45 min [7.5–56.8 min], angle 8.2°C [5.1–42.5°C], MA 29.2 ± 9.7 mm, P < 0.001), with TF activation returning all but the angle (42.5 ± 7.6°C) to values similar to citrated samples (angle = 56.7 ± 10.4°C, P = 0.017). Collection using a syringe/needle method revealed a shorter R time for prewarmed samples only ( R time 4.7 ± 0.7 min, vs. 5.6 ± 0.8 min for vacuum‐collected samples, P = 0.008). Conclusions Even in the absence of exogenous activators, CA has an impact on canine TEG results. The effects of rest temperatures and sample collection technique on TEG appear to be minimal.