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The HemoCue ® for point‐of‐care hemoglobin measurement and packed cell volume estimation in cats
Author(s) -
Posner Lysa P.,
Erb Hollis N.,
Gleed Robin D.
Publication year - 2005
Publication title -
journal of veterinary emergency and critical care
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.886
H-Index - 47
eISSN - 1476-4431
pISSN - 1479-3261
DOI - 10.1111/j.1476-4431.2005.04034.x
Subject(s) - hemoglobin , medicine , cats , coefficient of variation , point of care , limits of agreement , nuclear medicine , zoology , chromatography , pathology , biology , chemistry
Objective: To determine conversion formulas so that values from the HemoCue ® hemoglobinometer (Hb HQ ) could be equated to hemoglobin concentrations measured by the gold‐standard cyanomethemoglobin (Hb CY ) method and used for estimation of packed cell volume (PCV) in cats. Study design: Prospective, in vitro . Animals: Twelve healthy, adult, client‐owned cats. Interventions: The PCV of 12 parent blood samples was manipulated between ∼3 and ∼80% by removing or adding autologous plasma. Hemoglobin was measured by the Hb CY method at a university clinical pathology laboratory and by the azidemethemoglobin method in a Hb HQ . PCV was determined by micro‐centrifugation. Measurements and main results: Hemoglobin varied from 1–26 g/dL. Repeated‐measures regression of Hb CY on Hb HQ demonstrated that the Y ‐intercept was not different from zero. When the regression was repeated, forcing the line through the origin the coefficient for converting Hb HQ to Hb CY was not significantly different from 1.0 (adjusted R 2 =99.7%). The conversion formula was Hb CY =Hb HQ . Using similar methods, the conversion formula for estimating PCV was PCV=3.1 Hb HQ (adjusted R 2 =99.8%). Conclusion: Hemoglobin concentration measured by the HemoCue ® was indistinguishable from the (Hb CY ) method in feline blood over a wide range of hemoglobin concentrations. This study demonstrates that in cats PCV=3.1Hb HQ . Clinical relevance: The HemoCue ® is useful for point‐of‐care hemoglobinometry and for estimating PCV in cats.