Premium
Rabbit corneal endothelial cell membrane potential
Author(s) -
Wigham Chris C.,
Green Keith,
Hodson Stuart
Publication year - 1993
Publication title -
ophthalmic and physiological optics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.147
H-Index - 66
eISSN - 1475-1313
pISSN - 0275-5408
DOI - 10.1111/j.1475-1313.1993.tb00476.x
Subject(s) - lucifer yellow , microelectrode , biophysics , depolarization , ouabain , potassium , sodium , membrane potential , chemistry , extracellular , cell membrane , membrane , cell , intracellular , biochemistry , biology , gap junction , electrode , organic chemistry
Membrane potential of rabbit corneal endothelial cells measured using microelectrodes was − 29.3 ± 0.8 mV, n = 45. (mean ± SEV1). Histological location of Lucifer Yellow dye iontophoresed out of the microelectrode confirmed that the microelectrode was located intracellularly, The Lucifer Yellow diffused five to six cell diameters away from the impaled cell indicating endothelial cell coupling. Depolarization by ouabain (10 −4 M) and high extracellular potassium (potassium for sodium substitution) showed the cells to be responsive to changes in the bathing solution whilst impaled, that the cell membrane is more permeable to potassium than sodium and that membrane bound Na + ‐K + ‐ATPase activity generates the transmembrane electrolyte gradients.