Open AccessMonitoring of cellular senescence by DNA‐methylation at specific CpG sites
Author(s) -
Koch Carmen M.,
Joussen Sylvia,
Schellenberg Anne,
Lin Qiong,
Zenke Martin,
Wagner Wolfgang
Publication year - 2012
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/j.1474-9726.2011.00784.x
Subject(s) - biology , senescence , dna methylation , epigenetics , cpg site , methylation , microbiology and biotechnology , population , cellular senescence , genetics , dna , computational biology , phenotype , gene expression , gene , demography , sociology
Summary Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long‐term culture based on continuous DNA‐methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture.