Open AccessAmbient temperature influences aging in an annual fish ( Nothobranchius rachovii )
Author(s) -
Hsu ChinYuan,
Chiu YaChi
Publication year - 2009
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/j.1474-9726.2009.00525.x
Subject(s) - biology , catalase , superoxide dismutase , oxidative stress , reactive oxygen species , lipofuscin , senescence , lipid peroxidation , survivorship curve , bioenergetics , mitochondrion , biochemistry , microbiology and biotechnology , genetics , cancer
Summary Extending lifespan by lowering ambient temperature in the habitat has been shown in a variety of organisms. Its mechanism, however, remains elusive. In this study, we examined the survivorship and the aging process of the annual fish ( Nothobranchius rachovii ) reared under high (30 °C), moderate (25 °C) and low (20 °C) ambient temperatures. The results showed that low ambient temperatures prolong survivorship, whereas high ambient temperatures shorten survivorship. At low ambient temperature, expression of senescence‐associated β‐galactosidase, lipofuscin, reactive oxygen species, lipid peroxidation, protein oxidation, mitochondrial density and ADP/ATP ratio were reduced compared with those reared at high and moderate temperatures, whereas catalase activity, Mn‐superoxide dismutase activities, mitochondrial membrane potential and the levels of ATP, ADP, Sirt1 and Forkhead box O expression were elevated. The expression levels of Hsp70 and CIRP showed no significant difference under any of the ambient temperatures tested. We concluded that cellular metabolism, energy utilization and gene expression are altered at lower ambient temperature, which is associated with the extension of lifespan of the annual fish.