Open AccessSIRT1 transgenic mice show phenotypes resembling calorie restriction
Author(s) -
Bordone Laura,
Cohen Dena,
Robinson Ashley,
Motta Maria Carla,
Van Veen Ed,
Czopik Agnieszka,
Steele Andrew D.,
Crowe Hayley,
Marmor Stephen,
Luo Jianyuan,
Gu Wei,
Guarente Leonard
Publication year - 2007
Publication title -
aging cell
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.103
H-Index - 140
eISSN - 1474-9726
pISSN - 1474-9718
DOI - 10.1111/j.1474-9726.2007.00335.x
Subject(s) - transgene , biology , genetically modified mouse , phenotype , calorie restriction , sirtuin , sirtuin 1 , endocrinology , adipokine , longevity , medicine , transgenesis , genetics , leptin , gene , obesity , downregulation and upregulation , embryogenesis , reproductive biology , acetylation
Summary We generated mice that overexpress the sirtuin, SIRT1. Transgenic mice have been generated by knocking in SIRT1 cDNA into the β‐actin locus. Mice that are hemizygous for this transgene express normal levels of β‐actin and higher levels of SIRT1 protein in several tissues. Transgenic mice display some phenotypes similar to mice on a calorie‐restricted diet: they are leaner than littermate controls; are more metabolically active; display reductions in blood cholesterol, adipokines, insulin and fasted glucose; and are more glucose tolerant. Furthermore, transgenic mice perform better on a rotarod challenge and also show a delay in reproduction. Our findings suggest that increased expression of SIRT1 in mice elicits beneficial phenotypes that may be relevant to human health and longevity.