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UVB irradiation down‐regulates type XVI collagen expression in mouse and human skin
Author(s) -
Abe Hiroyuki,
Tajima Shingo
Publication year - 2012
Publication title -
journal of cosmetic dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.626
H-Index - 44
eISSN - 1473-2165
pISSN - 1473-2130
DOI - 10.1111/j.1473-2165.2012.00612.x
Subject(s) - photoaging , hairless , human skin , extracellular matrix , messenger rna , microbiology and biotechnology , type i collagen , collagen, type i, alpha 1 , chemistry , in vivo , reverse transcription polymerase chain reaction , real time polymerase chain reaction , biology , biochemistry , endocrinology , gene , genetics
Summary Background Type XVI collagen is a member of the fibril‐associated collagens with interrupted triple helices; however, its function or regulation remain unclear. Aims This study is to examine the effect of ultraviolet B (UVB) or photoaging on type XVI collagen expression in various cultured cells, mouse, and human skin. Methods The level of α1 (XVI) collagen mRNA was determined by quantitative real‐time reverse transcriptase‐polymerase chain reaction and the localization of type XVI collagen in normal human skins was detected by theα1 (XVI) collagen polypeptide antibody. Results Exposure of keratinocytes resulted in suppression of mRNA level in a dose‐ and time‐dependent manner and in normal fibroblasts or organotypic cocultures was also inhibited. Expression level in hairless mouse skin was decreased by UVB exposure. Messenger RNA level of human skins in the sun‐protected area appeared to be greater than that in the sun‐exposed area. Sun‐protected and sun‐exposed normal skin taken from young subjects showed positive immunoreactivities with the anti‐α1 (XVI) collagen antibody in the subepidermal region, whereas sun‐exposed skin from elderly subjects exhibited negative immunoreaction. Conclusions Reduction of type XVI collagen by UVB irradiation in vitro and in vivo may be related to the alteration of extracellular matrix in the photodamaged skin.