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Effect of DHA + EPA on oxidative stress and apoptosis induced by ischemia‐reperfusion in rat kidneys
Author(s) -
Ajami Marjan,
Davoodi Sayed Hossein,
Habibey Rouhollah,
Namazi Nasim,
Soleimani Mansoureh,
PazokiToroudi Hamidreza
Publication year - 2013
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2012.01066.x
Subject(s) - malondialdehyde , oxidative stress , superoxide dismutase , creatinine , apoptosis , chemistry , ischemia , kidney , endocrinology , medicine , reperfusion injury , pharmacology , nephrectomy , biochemistry
Abstract Apoptosis, as well as necrosis, has an important role in post‐ischemic renal pathology. The effect of pretreatment with D ocosahexaenoic acid+ E icosapentaenoic acid ( DHA + EPA ) on renal injury and apoptotic protein expression was evaluated. Right nephrectomy was completed on male W istar rats (255–300 g). The rats received DHA + EPA (200 mg/kg/day) of distilled water orally for 14 days before ischemia reperfusion ( IR ) or sham operation. A total of 81 rats were divided into three main groups with 6, 24 and 48 h of post‐operation or reperfusion period. Serum creatinine ( SC r), BUN , creatinine clearance ( CC r) and fractional excretion of sodium ( FE N a ) were measured. Tissue levels of malondialdehyde ( MDA ), superoxide dismutase ( SOD ) and catalase ( CAT ) activities, Bax and Bcl‐2 protein expressions and renal histological injury were determined. SC r, BUN and FE N a increased 6–48 h of reperfusion ( P < 0.01). Tissue MDA content and Bax expression increased ( P < 0.01) and CAT and SOD activities decreased ( P < 0.05) in the IR group. DHA + EPA decreased SC r and BUN , FE N a , tissue MDA levels ( P < 0.05 vs. IR ) and increased CAT and SOD activities and B cl‐2 expression ( P < 0.05 vs. IR ) for 6–48 h after ischemia. IR induced mild (6 h, P < 0.05) and severe (24–48 h, P < 0.01) tissue damage. Mild‐to‐moderate tissue damage was observed in DHA + EPA groups from 6 to 48 h of reperfusion period ( P < 0.05 vs. IR , 24–48 h). In conclusion, the results suggest that pre‐ischemic exposure to DHA + EPA could improve the outcome of early graft function by inhibition of IR ‐induced oxidative stress and apoptosis.