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Modulation of bone turnover in orchidectomized rats treated with raloxifene and risedronate
Author(s) -
Khedr Naglaa F.,
ElAshmawy Nahla E.,
ElBahrawy Hoda A.,
Haggag Ali A.,
ElAbd Eman E.
Publication year - 2013
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2012.01047.x
Subject(s) - endocrinology , medicine , bone remodeling , raloxifene , osteoporosis , osteocalcin , bone mineral , deoxypyridinoline , femur , alkaline phosphatase , tibia , bone density , chemistry , surgery , tamoxifen , biochemistry , cancer , breast cancer , enzyme
Osteoporosis is a reduction in bone mineral density ( BMD ). It develops less often in men than in women. This study aimed to evaluate the bone protective effects of raloxifene ( RAL ), risedronate ( RIS ), and their combination on osteoporotic male rats. Forty male W ister rats (12 weeks) were randomly divided into five groups: sham‐operated group ( n = 8), orchidectomized ( ORX ) group ( n = 7), RAL group ( n = 9), RIS group ( n = 7) and RAL + RIS group ( n = 7). RAL was orally administered at 3 mg/kg three times/week, and RIS was given subcutaneously at 5 μg/kg, twice weekly. After 6 weeks of treatment, serum cathepsin‐K, alkaline ( ALP ) and acid phosphatase activities, serum osteocalcin, serum Ca 2+ , and Pi were determined. Urinary Ca 2+ and deoxypyridinoline levels, BMD , and Ca 2+ content of femur ash were estimated. Histochemical localization of ALP activity of tibia and histomorphometry was examined. As compared to sham, ORX rats showed a significant increase in bone turnover markers, and histochemical activity of ALP was increased markedly in proximal tibia of ORX rats, whereas BMD and Ca 2+ content of femur ash were reduced after ORX . These changes were modulated after treatment with RAL and RIS or both to ORX rats; BMD of femur was improved by each treatment, and bone turnover markers were reduced as compared to ORX vehicle group. We concluded that orchidectomy induced osteoporosis and increased bone turnover in male rats because of withdrawal of sex hormones. Both RAL and RIS could treat osteoporosis in ORX rats; they reduced bone turnover markers and maintained BMD .