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The effect of nitroglycerin tolerance on oxidative stress and anaerobic sulfur metabolism in rat tissues
Author(s) -
Dudek Magdalena,
Bilska Anna,
Bednarski Marek,
Iciek Małgorzata,
Kwiecień Inga,
SokołowskaJeżewicz Maria,
Filipek Barbara,
Włodek Lidia
Publication year - 2010
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2009.00728.x
Subject(s) - rhodanese , catalase , chemistry , glutathione , oxidative stress , endocrinology , malondialdehyde , medicine , superoxide dismutase , nitric oxide , glutathione peroxidase , metabolism , kidney , thiol , biochemistry , biology , enzyme
The present results indicated that tolerance to nitroglycerin (glycerin trinitrate, GTN) increased the hepatic and renal level of reactive oxygen species, malondialdehyde, and glutathione peroxidase (PO x ) and decreased superoxide dismutase activity, whereas non‐protein thiols remained unchanged in both organs. In the liver (but not in the kidney) glutathione S ‐transferase (GST), catalase and rhodanese activities decreased and the sulfane sulfur level also dropped. Unlike in the liver, γ‐glutamyl transpeptidase (γGT) activity in the kidney declined. On the other hand, hepatic S‐nitrosothiols (SNT) rose while renal SNT declined. As no concomitant changes in the nitric oxide (NO) level were observed in either organ, the results suggested that under nitroglycerin tolerance NO was stored in the liver in the form of SNT. In conclusion, the results obtained in the liver and kidney confirm the involvement of oxidative stress in the pathomechanism of GTN tolerance, and reveal the diverse effects of this phenomenon on the γGT and GST activity and SNT level in both organs. We observed for the first time that GTN tolerance could be accompanied by the disruption of hepatic anaerobic cysteine metabolism, associated with sulfane sulfur and rhodanese activity.