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Y27632, a Rho‐activated kinase inhibitor, normalizes dysregulation in alpha1‐adrenergic receptor‐induced contraction of Lyon hypertensive rat artery smooth muscle
Author(s) -
Freitas Maria Regina,
Eto Masumi,
Kirkbride Jason A.,
Schott Christa,
Sassard Jean,
Stoclet JeanClaude
Publication year - 2009
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2008.00658.x
Subject(s) - myosin light chain phosphatase , medicine , rhoa , endocrinology , sma* , contraction (grammar) , myosin light chain kinase , myosin , rho associated protein kinase , phenylephrine , muscle contraction , contractility , phosphatase , chemistry , rho kinase inhibitor , agonist , phosphorylation , receptor , signal transduction , biochemistry , mathematics , combinatorics , blood pressure
RhoA‐activated kinase (ROK) is involved in the disorders of smooth muscle contraction found in hypertension model animals and patients. We examined whether the α1‐adrenergic receptor agonist‐induced ROK signal is perturbed in resistance small mesentery artery (SMA) of Lyon genetically hypertensive (LH) rats, using a ROK antagonist, Y27632. Smooth muscle strips of SMA and aorta were isolated from LH and Lyon normotensive (LN) rats. After Ca 2+ ‐depletion and pre‐treatment with phenylephrine (PE), smooth muscle contraction was induced by serial additions of CaCl 2 . In LH SMA Ca 2+ permeated cells to a lesser extent as compared with LN SMA, while CaCl 2 ‐induced contraction of LH SMA was greater than that of LN SMA, indicating a higher ratio of force to Ca 2+ in LH SMA contraction (Ca 2+ sensitization). No hyper‐contraction was observed in LH aorta tissues. Treatment of LH SMA with Y27632 restored both Ca 2+ permeability and Ca 2+ ‐force relationship to levels seen for LN SMA. In response to PE stimulation, phosphorylation of CPI‐17, a phosphorylation‐dependent myosin phosphatase inhibitor protein, and MYPT1 at Thr853, the inhibitory phosphorylation site of the myosin phosphatase regulatory subunit, was increased in LN SMA, but remained unchanged in LH SMA. These results suggest that the disorder in ROK‐dependent Ca 2+ permeability and Ca 2+ ‐force relationship is responsible for LH SMA hyper‐contraction. Unlike other hypertensive models, the ROK‐induced hyper‐contractility of LH SMA is independent of MYPT1 and CPI‐17 phosphorylation, which suggests that ROK‐mediated inhibition of myosin phosphatase does not affect SMA hyper‐contractility in LH SMA cells.