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Comparing reagents for efficient transfection of human primary myoblasts: FuGENE 6, Effectene and ExGen 500
Author(s) -
Arnold AnneSophie,
Laporte Vincent,
Dumont Serge,
AppertCollin Aline,
Erbacher Patrick,
Coupin Gilliane,
Levy Rachel,
Poindron Philippe,
Gies JeanPierre
Publication year - 2006
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2005.00344.x
Subject(s) - transfection , myocyte , cytotoxicity , reagent , genetic enhancement , primary cell , chemistry , cell culture , microbiology and biotechnology , cell , biology , biochemistry , gene , in vitro , genetics
This study compared three different synthetic reagents (FuGENE 6, Effectene and ExGen 500) for the transfection of human primary myoblasts. We examined the efficiency, cytotoxicity and size of the complexes formed in the presence of different amounts of vector and DNA and with variable amounts of serum. Transfection rates were relatively high for primary cells, especially with FuGENE 6 (20%), which appeared to be the best transfection reagent for these cells, even in the presence of 10% serum. Cultured human myoblasts are an interesting tool for studying neuromuscular diseases and are potentially useful for myoblast transfer therapy studies. Moreover, the efficiency of these transfection reagents in a medium containing 10% serum is promising for possible gene therapy protocols for muscle diseases.