Premium
Effect of lipopolysaccharide treatment on neurogenic contraction and noradrenaline release in rat arteries
Author(s) -
Ohlmann Patrick,
Martínez M. Carmen,
Bucher Bernard,
Andriantsitohaina Ramaroson,
Muller Bernard,
Schneider Francis,
Stoclet JeanClaude
Publication year - 2000
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2000.tb00445.x
Subject(s) - mesenteric arteries , endocrinology , contraction (grammar) , medicine , yohimbine , nitric oxide synthase , stimulation , nitric oxide , lipopolysaccharide , arginine , artery , chemistry , antagonist , receptor , biochemistry , amino acid
— In the present study, contractile responses and [ 3 H]‐noradrenaline overflow evoked by electrical field stimulation were assessed, respectively, in the small mesenteric artery and in tail artery removed from rats pre‐treated with either saline or lipopolysaccharide (LPS). In small mesenteric arteries, LPS treatment did not significantly modify the contractile responses elicited by electrical stimulation, in the absence or in the presence of L‐arginine. However, in arteries removed from rats treated with LPS, L‐arginine addition produced relaxation of vessels pre‐contracted with noradrenaline. The amplification of neurogenic contraction by the nitric oxide (NO) synthase inhibitor Nω‐nitro‐L‐arginine methyl ester (L‐NAME) was similar in arteries removed from saline and LPS‐infused rats. In mesenteric arteries, LPS treatment suppressed the potentiation of the neurogenic responses by the α 2 ‐adrenoceptor antagonist, yohimbine and by the inhibitor of neuronal uptake of noradrenaline, cocaine. In rat tail artery exposed to L‐arginine, LPS treatment produced an increase in [3H]‐nora‐drenaline overflow evoked by electrical stimulation. Altogether, these data suggest that an enhanced noradrenaline release from sympathetic nerves, probably resulting from inhibition of the modulatory effect of both prejunctional α 2 ‐adrenoceptors and neuronal uptake mechanism, may play a role in the preservation of neurogenic response after LPS treatment despite evidence of the induction of NO synthase.