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KR 31372, a benzopyran derivative, inhibits oxidized LDL‐stimulated proliferation and migration of vascular smooth muscle cells
Author(s) -
Kim Hyun Hee,
Ha Hun Joo,
Kim SunOk,
Kim SooKyung,
Yoo SungEun,
Hong Ki Whan
Publication year - 2000
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.2000.tb00429.x
Subject(s) - chemistry , probucol , platelet derived growth factor receptor , tbars , vascular smooth muscle , dna synthesis , endocrinology , incubation , thiobarbituric acid , medicine , antioxidant , intracellular , oxidative stress , biochemistry , microbiology and biotechnology , lipid peroxidation , growth factor , biology , dna , smooth muscle , receptor
— KR 31372 is a benzopyran derivative. Both [ 3 H]thymidine incorporation and migrations (chemotactic and wound‐edge) of cultured smooth muscle cells (SMCs) were greatly stimulated by oxidized low‐density lipoprotein (LDL). These effects were significantly suppressed by KR 31372 (10 −7 ‐ 10 −6 M) and PDGF‐BB antibody (10 −8 ‐ 10 −6 M). Preincubation with KR 31372 led to a decrease in the synthesis of PDGF‐BB‐like immunoreactivity (PDGF‐BB‐LI) that had been stimulated by oxidized LDL. Otherwise, KR 31372 and probucol strongly inhibited the production of thiobarbituric acid reactive substances (TBARS) caused by the incubation of LDL with Cu 2+ ion, and significantly reduced the intracellular oxidative stress when stimulated with H 2 O 2 . Taken together, it is suggested that KR 31372 may inhibit the oxidized LDL‐stimulated syntheses of DNA and PDGF‐BB, and migration of the SMCs, in part, via the antioxidant activity.