Premium
Monoclonal antibody fluorescent polarisation immunoassay versus 125 Iode labelled ligand radioimmunoassay for the measurement of cyclosporine concentrations in whole blood
Author(s) -
Montes C.,
Burtin P.,
JacqzAigrain E.
Publication year - 1991
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.1991.tb00742.x
Subject(s) - radioimmunoassay , monoclonal antibody , immunoassay , chemistry , whole blood , fluorescence polarization immunoassay , kidney , monoclonal , chromatography , quantitative analysis (chemistry) , antibody , medicine , immunology , biochemistry
Summary— Monoclonal antibody fluorescent polarisation immunoassay for cyclosporine in whole blood was first evaluated. Inter‐ and intra‐assay CVs were < 7%. We also compared concentrations measured by 125 I‐RIA and FPIA in specimens obtained from kidney and bone marrow transplanted patients. FPIA correlated well with 125 I‐RIA (slope = 1.03, r = 0.989, n = 58) over a wide range of concentrations (44–984 ng/ml). However, an additive bias estimated by the mean difference in cyclosporine concentrations between the 2 readings (44.1 ± 44.3 ng/ml), led to overestimation of cyclosporine concentrations measured by FPIA. The monoclonal FPIA kit is therefore a rapid and reproductive method to monitor cyclosporine concentrations in whole blood. However, FPIA and 125 I‐RIA are not interchangeable and the therapeutic range of cyclosporine measured by FPIA should be defined.