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QUANTITATIVE ANALYSIS OF SERUM METHOHEXITAL BY GLC USING CAPILLARY COLUMN AND NITROGEN‐SELECTIVE DETECTION
Author(s) -
NORMAND Y.,
VILLEPOIX C.,
ATHOUEL A.,
KERGUERIS M.F.,
BOURIN M.,
LAROUSSE C.
Publication year - 1988
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.1988.tb00655.x
Subject(s) - methohexital , chromatography , detection limit , chemistry , capillary action , pharmacokinetics , anesthesia , medicine , materials science , pharmacology , composite material , propofol
Summary— A gas chromatographic method for routine quantitation of methohexital in plasma samples is reported. One‐step extraction in organic phase, the use of a fused silica capillary column, and nitrogen‐selective detection permit simple, precise, and sensitive determination of methohexital in plasma. A linear relationship is described between peak height ratio and methohexital concentrations ranging from 0.125 to 50.0 μg/ml ( r =0.998). The sensitivity limit of the assay was 6 ng/ml in plasma. No interfering peak was observed with numerous other drugs. The procedure was successfully applied to the determination of pharmacokinetic parameters of methohexital after IV administration or continuous infusion in a child and an adult.