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RAPID GAS‐CHROMATOGRAPHIC ASSAY OF BUPIVACAINE IN PLASMA
Author(s) -
NORMAND Y.,
ATHOUEL A.,
BLANLOEIL Y.,
VILLEPOIX C.,
MELCHIOR J.C.,
KERGUERIS M.F.,
BOURIN M.,
LAROUSSE C.
Publication year - 1987
Publication title -
fundamental and clinical pharmacology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.655
H-Index - 73
eISSN - 1472-8206
pISSN - 0767-3981
DOI - 10.1111/j.1472-8206.1987.tb00580.x
Subject(s) - chromatography , chemistry , human plasma , gas chromatography , bupivacaine , extraction (chemistry) , plasma , capillary action , high performance liquid chromatography , capillary gas chromatography , local anesthetic , selectivity , anesthesia , materials science , medicine , organic chemistry , physics , quantum mechanics , composite material , catalysis
Summary— A method for estimating bupivacaine concentration in human plasma by capillary gas‐chromatography using solid injection and nitrogen‐specific detection is described. Etidocaine, another anilidetype local anesthetic was used as internal standard and added to the sample before single‐step extraction with diethylether. This method demonstrates high sensitivity (6 ng/ml plasma) and combines selectivity, rapidity, and simplicity. Results of this procedure correlate well with those obtained by an HPLC method.