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Oxygen transfer coefficient and the kinetic parameters of exo‐polygalacturonase production by A spergillus flavipes FP ‐500 in shake flasks and bioreactor
Author(s) -
Gómez Sánchez C.E.,
MartínezTrujillo A.,
Aguilar Osorio G.
Publication year - 2012
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2012.03313.x
Subject(s) - bioreactor , laboratory flask , fermentation , chemistry , biomass (ecology) , volume (thermodynamics) , chemostat , food science , chromatography , botany , biology , bacteria , organic chemistry , ecology , physics , quantum mechanics , genetics
Aims To evaluate and compare the sensitivity of E xo‐ P G production and kinetic parameters of A spergillus flavipes FP ‐500 to oxygen transfer condition in shake flasks and bioreactor. Methods and Results A spergillus flavipes FP ‐500 was grown on pectin as carbon source in shake flasks and bioreactor at different oxygen transfer conditions. The volumetric coefficient of oxygen transfer ( k L a ) was modified by changing both, the flask size/medium volume ratio and the agitation speed. Higher biomass concentration, E xo‐ PG activity, maximum specific growth rate and yield coefficient were obtained in bioreactor at higher k L a value. A strong correlation was found between biomass, E xo‐ PG activity and growth‐associated product coefficient to k L a in bioreactor but does not in shake flasks. The mathematical model provided a good description of growth, pectin consumption and E xo‐ PG production in submerged batch cultures carried out in bioreactor. Conclusions Biomass concentration, E xo‐ PG activity and their kinetics of A spergillus flavipes FP ‐500 were strongly influenced by oxygen transfer condition and cultivation system. Significance and Impact of Study The production of enzymes by fungal fermentation is strictly aerobic and understanding the influence of oxygen transfer condition on the production kinetic is of vital importance in order to design, optimize and translate bioprocesses to industrial scale.