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Characterization and antagonistic properties of S treptomyces strains isolated from S aharan soils, and evaluation of their ability to control seedling blight of barley caused by F usarium culmorum
Author(s) -
Yekkour A.,
Sabaou N.,
Zitouni A.,
Errakhi R.,
Mathieu F.,
Lebrihi A.
Publication year - 2012
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2012.03312.x
Subject(s) - fusarium culmorum , biology , seedling , fusarium , blight , fungicide , agronomy , horticulture , biological pest control , botany
During a screening for potential plant disease control actinomycetes, a total of 133 strains were isolated from S aharan soil samples of seven A lgerian regions by dilution technique on chitin‐vitamins agar medium. Screening for antagonistic properties using streak assay method showed that 25% of isolates demonstrated strong activities against a wide range of plant pathogenic fungi. Due to their strong anti‐ F usarium activities, six of these isolates were selected and subsequently related to S treptomyces species by polyphasic analysis. These isolates were evaluated for their biocontrol ability against F usarium culmorum , a serious pathogenic fungus of cereals crops related to damping‐off and seedling blight resulting in yield loss. Barley seeds were chosen as cereal plant model. Surface bacterized seeds with TW 3, RI 3 and TW 2 strains expressed the highest performances and permit to reduce significantly both the disease occurrence on seedlings (62–76%) and the extent of seedling blight symptoms (over than 95%). However, a negative effect on plant establishment was observed for RI 3 treatment. Significance and Impact of the Study The genus F usarium is considered to be one of the most problematic phytopathogenic fungi for crop culture worldwide. Inside this genus, F. culmorum is the aetiological agent of seedling blight in various monocotyledonous plants such as barley and cause extensive yield and quality losses in humid and semi‐humid regions. Biological control may be a successful alternative to chemical control, particularly with the controversy surrounding the use of the fungicides and the limited obtained results to control F . culmorum . This study highlights the effectiveness of some antagonistic S treptomyces isolated from A lgerian S aharan soils to control F . culmorum by the reduction in disease occurrence and disease severity suggesting their use on microbial biocontrol formulation against soilborne diseases.