Premium
Extended‐spectrum beta‐lactamase‐producing Escherichia coli in turkey meat production farms in the Czech Republic: National survey reveals widespread isolates with bla SHV‐12 genes on IncFII plasmids
Author(s) -
Dolejska M.,
Matulova M.,
Kohoutova L.,
Literak I.,
Bardon J.,
Cizek A.
Publication year - 2011
Publication title -
letters in applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.698
H-Index - 110
eISSN - 1472-765X
pISSN - 0266-8254
DOI - 10.1111/j.1472-765x.2011.03099.x
Subject(s) - biology , escherichia coli , plasmid , beta lactamase , flock , microbiology and biotechnology , klebsiella pneumoniae , enterobacteriaceae , gene , genetics , ecology
Aim: The occurrence and epidemiology of extended‐spectrum beta‐lactamase (ESBL)‐producing Escherichia coli in the environment of turkey farms in the Czech Republic were studied. Methods and Results: Extended‐spectrum beta‐lactamase‐producing E. coli isolates were found on 8 (20%) of 40 turkey farms surveyed. A total of 200 environmental smears were examined, and a total of 25 ESBL‐producing E. coli were isolated. These isolates were analysed using Xba I pulsed‐field gel electrophoresis and divided into nine pulsotypes. Most of the isolates harboured the gene bla SHV‐12 on a 40‐kb plasmid of the IncFII group with an identical Eco RV restriction profile. Indistinguishable or clonally related SHV‐12‐producing isolates belonging to the same pulsotypes were found at some unrelated farms. Conclusions: Widespread occurrence of ESBL‐producing E. coli isolates with bla SHV‐12 carried on IncFII plasmids in meat production flocks in the Czech Republic was demonstrated. Significance and Impact of the Study: Results indicate vertical transmission of ESBL‐producing E. coli within the turkey production pyramid. The study shows the risk of multiresistant ESBL‐producing bacteria and antibiotic‐resistance genes being transmitted to humans via the food chain.